Encapsulation of viable animal cells for hybrid bioartificial organs by the Biosil method

Giovanni Carturan; Giovanni Dellagiacoma; M. Rossi; R. Dal Monte; M. Muraca

doi:10.1117/12.279168

2 October 1997 Encapsulation of viable animal cells for hybrid bioartificial organs by the Biosil method

Giovanni Carturan, Giovanni Dellagiacoma, M. Rossi, R. Dal Monte, M. Muraca

Proceedings Volume 3136, Sol-Gel Optics IV; (1997) https://doi.org/10.1117/12.279168
Event: Optical Science, Engineering and Instrumentation '97, 1997, San Diego, CA, United States

Abstract

Gas phase silicon alkoxides react with the wet surface of mammalian cells, affording a stable and homogeneous layer of amorphous SiO₂ modified by Si-CH₃ and Si-H bonds. Layer thickness may be controlled by exposure time. The layer does not suppress cell viability or functionality, and may be applied to cells supported on a trapping network or to cell aggregates. H4-II-E-C3 rat hepatoma cells, Hep G2 human cancer cells and human fibroblasts on various supports were encapsulated by the SiO₂ layer and studied in terms of glucose utilization and ³H-leucin incorporation into secreted proteins. In the case of pancreatic islets, encapsulation was carried out without supports, so that original islet volume and features were maintained. In vitro results indicate preservation of vitality and function, as tested by insulin production.

Citation Download Citation

Giovanni Carturan, Giovanni Dellagiacoma, M. Rossi, R. Dal Monte, and M. Muraca "Encapsulation of viable animal cells for hybrid bioartificial organs by the Biosil method", Proc. SPIE 3136, Sol-Gel Optics IV, (2 October 1997); https://doi.org/10.1117/12.279168

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