Blood cell research using methods of microinterferometry

Gennady I. Kozinets; Julia K. Novoderzhkina; Elena A. Streletskaya; Gennady G. Levin; Gennady N. Vishnyakov

doi:10.1117/12.273652

2 May 1997 Blood cell research using methods of microinterferometry

Gennady I. Kozinets, Julia K. Novoderzhkina, Elena A. Streletskaya, Gennady G. Levin, Gennady N. Vishnyakov

Proceedings Volume 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology; (1997) https://doi.org/10.1117/12.273652
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Many biological objects, include blood cells, are optically transparent. They do not absorb light, and only change optical path length by variations of object's thickness or its refractive index variations. For visualization of phase information and obtaining quantitative properties of cells we use phase-shift interferometry. Cells are fixed by glutaraldehyde and mounted in immersion liquid for reduction the influences of refraction. Characteristic peculiarity of interferometric microscope are two channels of optical scheme: object and reference. Blood cell's interferogram contain the information about space distribution refractive index. For its reconstruction we use phase-shift method. This method includes record of four interferograms used different values of phase-shift of the reference wave. Many blood cells interferogram are obtained. The shape, size and density of cells are measured.

Citation Download Citation

Gennady I. Kozinets, Julia K. Novoderzhkina, Elena A. Streletskaya, Gennady G. Levin, and Gennady N. Vishnyakov "Blood cell research using methods of microinterferometry", Proc. SPIE 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, (2 May 1997); https://doi.org/10.1117/12.273652

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