Probing structure of blood plasma proteins with solvatochromic fluorescent probes based on Nile red and its derivatives

Gregory V. Kaler; Andrei I. Ivanov; Sergei V. Konev

doi:10.1117/12.273553

7 May 1997 Probing structure of blood plasma proteins with solvatochromic fluorescent probes based on Nile red and its derivatives

Gregory V. Kaler, Andrei I. Ivanov, Sergei V. Konev

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Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273553
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Uncharged long-wave fluorescent probes, Nile red and its derivatives varying in lipophilicity, were used for probing hydrophobic binding sites of human serum albumin (HSA) and lipoproteins (LP) in norm and pathology. The synchro-scan fluorescence spectra (synchronous scanning of both excitation and emission wavelengths at constant (Delta) (lambda) ) of the probes were studied in HSA solutions and in whole blood plasma. The parameters of the spectra were sensitive to pH-induced conformational NyieldsB transition in HSA. In blood plasma, each of the probes displayed a two-component synchro-scan spectrum revealing two pools of the dye bound to HSA (longer wavelength) and LP (shorter wavelength). The probe distribution between LP and HSA was also sensitive to NyieldsB transition. The LP/HSA probe distribution ratio was shown to increase significantly in certain pathologies, due to either hypoalbuminemia or lowered ligand-binding capacity of HSA. Also, spectral shifts were observed in the band of albumin-bound probe. The determination of the distribution parameter may be proposed as an informative and feasible diagnostic test.

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Gregory V. Kaler, Andrei I. Ivanov, and Sergei V. Konev "Probing structure of blood plasma proteins with solvatochromic fluorescent probes based on Nile red and its derivatives", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273553

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KEYWORDS

Plasma

Blood

Proteins

Luminescence

Pathology

Diagnostic tests

Absorption

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