Electrochemiluminescence at microelectrodes for biosensing

Rosemary L. Smith; YunTai Hsueh; Scott D. Collins; Jean-Charles Fiaccabrino; Milena Koudelka-Hep

doi:10.1117/12.269955

31 March 1997 Electrochemiluminescence at microelectrodes for biosensing

Rosemary L. Smith, YunTai Hsueh, Scott D. Collins, Jean-Charles Fiaccabrino, Milena Koudelka-Hep

Proceedings Volume 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications; (1997) https://doi.org/10.1117/12.269955
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

This paper presents a new method for obtaining highly efficient electrochemiluminescence (ECL) of tris(2,2'- bipyridine) ruthenium (TBR) in aqueous solutions and a biosensor which utilizes this method. An interdigitated, microelectrode array is employed with electrode widths and spacings of 5 micrometer. The microelectrode is supported on a silicon nitride coated silicon substrate and occupies 1 mm². Each microelectrode is 1 mm long and 5 microns wide. The diffusion enhancement produced by the microelectrode geometry, the small electrode spacing and the electrode material are all critical parameters for high ECL efficiency. ECL has been detected from TBR concentrations as low as 1 (mu) M, using a silicon PIN photodiode detector at room temperature. For biosensing applications, TBR is attached to the molecule of interest and ECL is then generated at the electrode surfaces. Cell configuration and the results of preliminary studies of the detection of TBR labeled DNA, attached to paramagnetic beads are presented.

Citation Download Citation

Rosemary L. Smith, YunTai Hsueh, Scott D. Collins, Jean-Charles Fiaccabrino, and Milena Koudelka-Hep "Electrochemiluminescence at microelectrodes for biosensing", Proc. SPIE 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications, (31 March 1997); https://doi.org/10.1117/12.269955

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