Reference and sensing pads for integrated optical immunosensors

Juerg Duebendorfer; Rino E. Kunz; E. Mader; Gert L. Duveneck; Markus Ehrat

doi:10.1117/12.259980

26 November 1996 Reference and sensing pads for integrated optical immunosensors

Juerg Duebendorfer, Rino E. Kunz, E. Mader, Gert L. Duveneck, Markus Ehrat

Proceedings Volume 2928, Biomedical Systems and Technologies; (1996) https://doi.org/10.1117/12.259980
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

For integrated optical immunosensors, a much more reliable interpretation of the sensor output signal can be achieved by introducing reference pads located near the sensing pads, in order to separate specific from nonspecific effects. Results of theoretical and experimental investigations are reported for immunosensors based on measuring changes of the effective refractive index due to the binding of analyte molecules. The emphasis is on the correction for temperature variations which is an important example of compensation for nonspecific effects. Using the binding of rabbit immunoglobulin to immobilized protein A as a regenerable model bioaffinity system, the influence of consecutive assay cycles on the two pads has been investigated. Experiments have been performed using sensor chips consisting of TiO₂ waveguiding films on fused silica and replicated polycarbonate substrates.

Citation Download Citation

Juerg Duebendorfer, Rino E. Kunz, E. Mader, Gert L. Duveneck, and Markus Ehrat "Reference and sensing pads for integrated optical immunosensors", Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); https://doi.org/10.1117/12.259980

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