Comparative study viruses with computer-aided phase microscope AIRYSCAN

Vladimir P. Tychinsky; Georgy E. Koufal; Elena V. Perevedentseva; Tatiana V. Vyshenskaia

doi:10.1117/12.260806

6 December 1996 Comparative study viruses with computer-aided phase microscope AIRYSCAN

Vladimir P. Tychinsky, Georgy E. Koufal, Elena V. Perevedentseva, Tatiana V. Vyshenskaia

Proceedings Volume 2926, Optical Biopsies and Microscopic Techniques; (1996) https://doi.org/10.1117/12.260806
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

Traditionally viruses are studied with scanning electron microscopy (SEM) after complicated procedure of sample preparation without the possibility to study it under natural conditions. We obtained images of viruses (Vaccinia virus, Rotavirus) and rickettsias (Rickettsia provazekii, Coxiella burnetti) in native state with computer-aided phase microscope airyscan -- the interference microscope of Linnik layout with phase modulation of the reference wave with dissector image tube as coordinate-sensitive photodetector and computer processing of phase image. A light source was the He-Ne laser. The main result is coincidence of dimensions and shape of phase images with available information concerning their morphology obtained with SEM and other methods. The fine structure of surface and nuclei is observed. This method may be applied for virus recognition and express identification, investigation of virus structure and the analysis of cell-virus interaction.

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Vladimir P. Tychinsky, Georgy E. Koufal, Elena V. Perevedentseva, and Tatiana V. Vyshenskaia "Comparative study viruses with computer-aided phase microscope AIRYSCAN", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); https://doi.org/10.1117/12.260806

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KEYWORDS

Microscopes

Scanning electron microscopy

Viruses

Phase shift keying

Atomic force microscopy

Helium neon lasers

Image processing

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