Flow cytometric studies of cellular uptake of PII, m-THPP and m-THPC: kinetics and cell density dependence

F. D. Hanisch; Harald B. Steen; Johan Moan

doi:10.1117/12.260756

4 December 1996 Flow cytometric studies of cellular uptake of PII, m-THPP and m-THPC: kinetics and cell density dependence

F. D. Hanisch, Harald B. Steen, Johan Moan

Author Affiliations +

Proceedings Volume 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II; (1996) https://doi.org/10.1117/12.260756
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

The uptake of the photosensitizers Photofrin II (PII), meso- tetra-hydroxyphenyl-porphyrin (m-THPP) and meso-tetra- hydroxyphenyl-chlorin (m-THPC) in REH-cells was studied by flow cytometry (FCM). The uptake kinetics were studied with and without metabolic inhibitors. The uptake of PII after 60 min incubation was roughly 50% lower in the presence of the metabolic inhibitors, suggesting a partly active uptake mechanism for PII in REH-cells. The accumulation of m-THPP and m-THPC was not reduced by the metabolic inhibitors. Incubating PII-loaded cells for 60 minutes in sensitizer- free medium reduced the fluorescence intensity by approximately 60%, but had only a minor impact on the fluorescence intensity of cells incubated with m-THPP or m- THPC, even when the washing medium contained 10% serum. The uptake per cell was 9.5, 16, and 11 times higher at 10⁵ than at 10⁷ cells ml^-1 for PII, m-THPP and M- THPC in PBS, respectively. This was not solely due to depletion of dye from the medium, since the extracellular sensitizer-concentrations decreased less than 50%. Medium from suspensions of high cell density inhibited the uptake of the sensitizers in low cell density suspensions, indicating that the inhibition may be due to factors secreted by the cells.

Citation Download Citation

F. D. Hanisch, Harald B. Steen, and Johan Moan "Flow cytometric studies of cellular uptake of PII, m-THPP and m-THPC: kinetics and cell density dependence", Proc. SPIE 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II, (4 December 1996); https://doi.org/10.1117/12.260756

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
11 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Light scattering

Information operations

Proteins

Plasma

Scanning electron microscopy

Flow cytometry

Show All Keywords

Keywords/Phrases

Search In:

Publication Years