Application Of Laser Fluorimetry To Enzyme-Linked Immunoassay

William D. Hinsberg III; Kristin H. Milby; Steven D. Lidofsky; Richard N. Zare

doi:10.1117/12.965825

8 September 1981 Application Of Laser Fluorimetry To Enzyme-Linked Immunoassay

William D. Hinsberg III, Kristin H. Milby, Steven D. Lidofsky, Richard N. Zare

Proceedings Volume 0286, Laser Spectroscopy for Sensitive Detection; (1981) https://doi.org/10.1117/12.965825
Event: 1981 Technical Symposium East, 1981, Washington, D.C., United States

Abstract

An enzyme-linked sandwich immunoassay for insulin is described. Horseradish peroxidase is employed as an enzyme label for antibody, and enzyme activity is measured via the fluorogenic substrate, p-hydroxyphenylacetic acid. The product is detected by excitation of fluorescence with the 325 nm line of a cw helium-cadmium ion laser on-line with reverse phase high performance liquid chromatography. The method requires a total incubation time of 45 minutes, and the limit of insulin detection is 1.1 μU/mℓ (6.6 pM). This assay is applicable to the analysis of human serum samples.

Citation Download Citation

William D. Hinsberg III, Kristin H. Milby, Steven D. Lidofsky, and Richard N. Zare "Application Of Laser Fluorimetry To Enzyme-Linked Immunoassay", Proc. SPIE 0286, Laser Spectroscopy for Sensitive Detection, (8 September 1981); https://doi.org/10.1117/12.965825

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