Measurement of the motion and surface topology of the living organisms using computer-aided laser microscopy

Alexander V. Tavrov; R. Masselink; H. H. Schreier; M. G. Vicker

doi:10.1117/12.233013

13 February 1996 Measurement of the motion and surface topology of the living organisms using computer-aided laser microscopy

Alexander V. Tavrov, R. Masselink, H. H. Schreier, M. G. Vicker

Author Affiliations +

Proceedings Volume 2729, Optical Velocimetry; (1996) https://doi.org/10.1117/12.233013
Event: Optical Velocimetry, 1995, Warsaw, Poland

Abstract

Stress factors can be visualized reliably by measurements of motion dynamics. Spatial portraits and temporal diagrams of impulses from two different kinds of cells -- Physarum and Dictyostelium discoideum -- are considered in the present work. Large numbers of biological applications require development of quantitative measurements in the dimensional range of classical optics. In order to apply this, we used a 3D RM 600 AUTOFOCUS Profilometer with spatial resolution of 1 micrometer². It allows measurement at the dynamical bands of 0.001 - 120 Hz. Perspectives and measured results as well as applications of another computer-aided microscopical technique are discussed.

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Alexander V. Tavrov, R. Masselink, H. H. Schreier, and M. G. Vicker "Measurement of the motion and surface topology of the living organisms using computer-aided laser microscopy", Proc. SPIE 2729, Optical Velocimetry, (13 February 1996); https://doi.org/10.1117/12.233013

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