Corneal collagen denaturation in laser thermokeratoplasty

Ralf Brinkmann; Juergen Kampmeier; Ulf Grotehusmann; Alfred Vogel; Norbert Koop; Mary Asiyo-Vogel; Reginald Birngruber

doi:10.1117/12.239611

7 May 1996 Corneal collagen denaturation in laser thermokeratoplasty

Ralf Brinkmann, Juergen Kampmeier, Ulf Grotehusmann, Alfred Vogel, Norbert Koop, Mary Asiyo-Vogel, Reginald Birngruber

Proceedings Volume 2681, Laser-Tissue Interaction VII; (1996) https://doi.org/10.1117/12.239611
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

In laserthermokeratoplasty (LTK) thermal denaturation and shrinkage of corneal collagen is used to correct hyperopia and astigmatism. In order to optimize dosimetry, the temperature at which maximal shrinkage of collagen fibrils occurs is of major interest. Since the exposure time in clinical LTK-treatment is limited to a few seconds, the kinetics of collagen denaturation as a rate process has to be considered, thus the time of exposure is of critical importance for threshold and shrinkage temperatures. We investigated the time-temperature correlation for corneal collagen denaturation within different time domains by turbidimetry of scattered HeNe laser probe light using a temperature controlled water bath and pulsed IR laser irradiation. In the temperature range of 60 degree(s)C to 95 degree(s)C we found an exponential relation between the denaturation time and temperature. For the typical LTK-treatment time of 2 s, a temperature of 95 degree(s)C is needed to induce thermal damage. Use of pulsed Holmium laser radiation gave significant scattering of HeNe laser probe light at calculated temperatures of around 100 degree(s)DC. Rate parameters according to the formalism of Arrhenius were fitted to these results. Force measurements showed the simultaneous onset of light scattering and collagen shrinkage.

Citation Download Citation

Ralf Brinkmann, Juergen Kampmeier, Ulf Grotehusmann, Alfred Vogel, Norbert Koop, Mary Asiyo-Vogel, and Reginald Birngruber "Corneal collagen denaturation in laser thermokeratoplasty", Proc. SPIE 2681, Laser-Tissue Interaction VII, (7 May 1996); https://doi.org/10.1117/12.239611

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