Use of intrinsic fluorescent signals for characterizing tissue metabolic states in health and disease

Britton Chance

doi:10.1117/12.237569

5 April 1996 Use of intrinsic fluorescent signals for characterizing tissue metabolic states in health and disease

Britton Chance

Proceedings Volume 2679, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy; (1996) https://doi.org/10.1117/12.237569
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

The large content of mitochondria in metabolizing cells, coupled with intrinsic NADH and flavoprotein signals makes these signals ideal for characterizing tissue metabolic states in health and disease. The first few millimeters of tissue are reached by the fluorescence excitation in the exposed surfaces of the cervix, bladder, rectum and esophagus, etc. Thus, extensive use has been made of fluorescent signals by a large number of investigators for tumor diagnosis from an empirical standpoint where the fluorescent signals are generally diminished in precancerous and cancerous tissue. This article reviews the biochemical basis for the fluorescent signals and points to a 'gold standard' for fluorescent signal examination involving freeze trapping and low temperature two- or three-dimensional high resolution fluorescence spectroscopy.

Citation Download Citation

Britton Chance "Use of intrinsic fluorescent signals for characterizing tissue metabolic states in health and disease", Proc. SPIE 2679, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy, (5 April 1996); https://doi.org/10.1117/12.237569

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