Optical biopsy fiber-based fluorescence spectroscopy instrumentation

Alvin Katz; Singaravelu Ganesan; Yuanlong Yang; Gui Chen Tang; Yury Budansky; Edward J. Celmer M.D.; Howard E. Savage; Stimson P. Schantz M.D.; Robert R. Alfano

doi:10.1117/12.237579

5 April 1996 Optical biopsy fiber-based fluorescence spectroscopy instrumentation

Alvin Katz, Singaravelu Ganesan, Yuanlong Yang, Gui Chen Tang, Yury Budansky, Edward J. Celmer M.D., Howard E. Savage, Stimson P. Schantz M.D., Robert R. Alfano

Author Affiliations +

Proceedings Volume 2679, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy; (1996) https://doi.org/10.1117/12.237579
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Native fluorescence spectroscopy of biomolecules has emerged as a new modality to the medical community in characterizing the various physiological conditions of tissues. In the past several years, many groups have been working to introduce the spectroscopic methods to diagnose cancer. Researchers have successfully used native fluorescence to distinguish cancerous from normal tissue samples in rat and human tissue. We have developed three generations of instruments, called the CD-scan, CD-ratiometer and CD-map, to allow the medical community to use optics for diagnosing tissue. Using ultraviolet excitation and emission spectral measurements on both normal and cancerous tissue of the breast, gynecology, colon, and aerodigestive tract can be separated. For example, from emission intensities at 340 nm to 440 nm (300 nm excitation), a statistically consistent difference between malignant tissue and normal or benign tissue is observed. In order to utilize optical biopsy techniques in a clinical setting, the CD-scan instrument was developed, which allows for rapid and reliable in-vitro and in-vivo florescence measurements of the aerodigestive tract with high accuracy. The instrumentation employs high sensitivity detection techniques which allows for lamp excitation, small diameter optical fiber probes; the higher spatial resolution afforded by the small diameter probes can increase the ability to detect smaller tumors. The fiber optic probes allow for usage in the aerodigestive tract, cervix and colon. Needle based fiber probes have been developed for in-vivo detection of breast cancer.

Citation Download Citation

Alvin Katz, Singaravelu Ganesan, Yuanlong Yang, Gui Chen Tang, Yury Budansky, Edward J. Celmer M.D., Howard E. Savage, Stimson P. Schantz M.D., and Robert R. Alfano "Optical biopsy fiber-based fluorescence spectroscopy instrumentation", Proc. SPIE 2679, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy, (5 April 1996); https://doi.org/10.1117/12.237579

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available