Optical diagnostics and mathematical models of intracellular protoplasmic mobility in amoeboid cells

Yury M. Romanovsky; Alexander V. Priezzhev; Dimitry A. Pavlov; Vladimir A. Teplov

doi:10.1117/12.239537

10 May 1996 Optical diagnostics and mathematical models of intracellular protoplasmic mobility in amoeboid cells

Yury M. Romanovsky, Alexander V. Priezzhev, Dimitry A. Pavlov, Vladimir A. Teplov

Proceedings Volume 2678, Optical Diagnostics of Living Cells and Biofluids; (1996) https://doi.org/10.1117/12.239537
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

A review of the optical methods designed for the study of intracellular protoplasmic streaming is presented. While in case of low streaming velocities (less than 10 mcm/s) the computer analysis of the images of moving protoplasm is effective, for higher velocities the laser Doppler microscopy has an advantage. With the help of the Doppler technique it is possible to determine the velocity profiles for nonstationary protoplasmic streamings in amoeboid cells. These data and also complex time changes of protoplasmic flow in response to external factors enable to determine and to specify parameters used for designing mathematical models of intracellular dynamics. As an example, results of experiments, modelling and simulations are presented for myxomycete plasmodium Physarum polycephalum which is a huge cell with typical amoeboid properties.

Citation Download Citation

Yury M. Romanovsky, Alexander V. Priezzhev, Dimitry A. Pavlov, and Vladimir A. Teplov "Optical diagnostics and mathematical models of intracellular protoplasmic mobility in amoeboid cells", Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); https://doi.org/10.1117/12.239537

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