Vectorial theory of confocal fluorescence

Colin J. R. Sheppard; Peter Torok

doi:10.1117/12.237488

10 April 1996 Vectorial theory of confocal fluorescence

Colin J. R. Sheppard, Peter Torok

Proceedings Volume 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III; (1996) https://doi.org/10.1117/12.237488
Event: Electronic Imaging: Science and Technology, 1996, San Jose, CA, United States

Abstract

Imaging in fluorescence microscopy is analyzed using a vectorial diffraction theory. Both conventional and confocal microscopy are considered. A fluorescent molecule is modeled as a radiating electric dipole. Two particular limiting cases are considered: the dipole can either freely rotate in space between excitation and emission, or is fixed in space. For each case, we average over all dipole orientations.

Citation Download Citation

Colin J. R. Sheppard and Peter Torok "Vectorial theory of confocal fluorescence", Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); https://doi.org/10.1117/12.237488

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