In situ off-axis holography of marine plankton

Eric Paul Krantz; John Watson; Peter R. Hobson; R. S. Lampitt; A. Rogerson

doi:10.1117/12.236066

25 March 1996 In situ off-axis holography of marine plankton

Eric Paul Krantz, John Watson, Peter R. Hobson, R. S. Lampitt, A. Rogerson

Proceedings Volume 2652, Practical Holography X; (1996) https://doi.org/10.1117/12.236066
Event: Electronic Imaging: Science and Technology, 1996, San Jose, CA, United States

Abstract

We present an off-axis transmission holographic technique for recording marine plankton in situ within a test tank of 36,000 ml with a pulsed laser in a 40 ns interval. The holographic plate is located in air and is therefore unaffected by aquatic conditions that may cause emulsion degradation (e.g. non-uniform swelling and surface contamination). The reference beam traverses a path in air only, and thus remains unaffected by dense concentrations of plankton. Third order aberrations, notably spherical aberration and astigmatism, are suppressed to yield an experimental resolution of 7 lp/mm (70 micrometers ) with a USAF 1951 target located 600 mm in water from the observation window. Plankton particle counts examined by real image reconstruction show a strong correlation with duplicate samples examined under a microscope.

Citation Download Citation

Eric Paul Krantz, John Watson, Peter R. Hobson, R. S. Lampitt, and A. Rogerson "In situ off-axis holography of marine plankton", Proc. SPIE 2652, Practical Holography X, (25 March 1996); https://doi.org/10.1117/12.236066

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