Laser-induced fluorescence studies on collagen, cholesterol, and chlorophyll a

Mihail-Lucian Pascu; N. Moise; S. Hogiu

doi:10.1117/12.228895

27 December 1995 Laser-induced fluorescence studies on collagen, cholesterol, and chlorophyll a

Mihail-Lucian Pascu, N. Moise, S. Hogiu

Proceedings Volume 2627, Optical Biopsies; (1995) https://doi.org/10.1117/12.228895
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

Results on laser induced fluorescence (LIF) properties of collagen, cholesterol, and chlorophyll a (chl a) are reported, when excited at 337.1 nm. The collagen and cholesterol fluorescence was studied in connection with atherosclerotic plaques diagnostics and/or removal within the cardiovascular system. The fluorescence signal excitation and collection were made using optical fibers, for liquid samples at concentrations between 0.1 mg/ml and 5.0 mg/ml for both collagen and cholesterol, their fluorescence lifetime being 5 nsec and 6 nsec, respectively. CHl a LIF concerned the stimulated emission of radiation considering the pigment as laser active medium. Chl a concentrations varied, in different solvents between 5 multiplied by 10^-3 M/l and 10^-5 M/l. For chl a at 0.62 mM/l and pumping photon densities higher than 5 multiplied by 10¹⁶ photons/cm² and pulse, fluorescence linewidth narrowing from 28 nm to 5 nm in ethanol and from 25 nm to 4 nm in DMSO were obtained. Lasing maxima at 672 nm in acetone, 674 nm in ethanol and 678 nm in DMSO were observed. At lasing conditions for chl a in DMSO (1mM/l) the measured fluorescence lifetime was 2 nsec instead of 5 nsec for normal fluorescence. The results on chl a stimulated emission show a lower lasing threshold at 10¹⁶ photons/cm² and pulse, than previously reported, if DMSO solvent is used.

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Mihail-Lucian Pascu, N. Moise, and S. Hogiu "Laser-induced fluorescence studies on collagen, cholesterol, and chlorophyll a", Proc. SPIE 2627, Optical Biopsies, (27 December 1995); https://doi.org/10.1117/12.228895

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KEYWORDS

Luminescence

Collagen

Laser induced fluorescence

Bioalcohols

Optical fibers

Curium

Cardiovascular system

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