Prospects for soft x-ray contact microscopy using laser plasmas as an x-ray source

Anthony D. Stead; Anton M. Page; Thomas W. Ford

doi:10.1117/12.220995

18 September 1995 Prospects for soft x-ray contact microscopy using laser plasmas as an x-ray source

Anthony D. Stead, Anton M. Page, Thomas W. Ford

Proceedings Volume 2523, Applications of Laser Plasma Radiation II; (1995) https://doi.org/10.1117/12.220995
Event: SPIE's 1995 International Symposium on Optical Science, Engineering, and Instrumentation, 1995, San Diego, CA, United States

Abstract

Since its invention, a major concern of those using a microscope has been to improve the resolution without the introduction of artifacts. While light microscopy carrier little risk of the introduction of artifacts, because the preparative techniques are often minimal, the resolution is somewhat limited. The advent of the electron microscope offered greatly improved resolution but since biological specimens require extensive preparation, the possiblity of causing structural damage to the specimen is also increased. The ideal technique for structural studies of biological specimens would enable hydrated material to be examined without any preparation and with a resolution equal to that of electron microscopy. Soft x-ray microscopy certainly enables living material to be examined and while the resolution does not equal that of electron microscopy it exceeds that attainable by light microscopy. This paper briefly reviews the limitations of light and electron microscopy for the biologist the various ways that soft x- rays might be used to image hydrated biological material. Consideration is given to the different sources that have been used for soft x-ray microscopy and the relative merits of laser-plasma sources are discussed.

Citation Download Citation

Anthony D. Stead, Anton M. Page, and Thomas W. Ford "Prospects for soft x-ray contact microscopy using laser plasmas as an x-ray source", Proc. SPIE 2523, Applications of Laser Plasma Radiation II, (18 September 1995); https://doi.org/10.1117/12.220995

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