Lifetime-based identification of single molecules

Joerg Enderlein; Rolf Krahl; Uwe Ortmann; Michael Wahl; Rainer Erdmann; Edgar O. Klose

doi:10.1117/12.208465

8 May 1995 Lifetime-based identification of single molecules

Joerg Enderlein, Rolf Krahl, Uwe Ortmann, Michael Wahl, Rainer Erdmann, Edgar O. Klose

Proceedings Volume 2388, Advances in Fluorescence Sensing Technology II; (1995) https://doi.org/10.1117/12.208465
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Our new developed time-correlated single photon counting electronics allows for continuous registration of time-resolved fluorescence signals in millisecond intervals. With this electronics, adapted fluorescence measurement system, we studied single molecule transitions through a focused laser beam. In our paper we present measurements on single rhodamine 6G- molecules and discuss data processing methods, using the full information of the time resolved measurement. The use of this information influences significantly the reliability of the detection of single molecule transits. Further developments and applications of this method are discussed.

Citation Download Citation

Joerg Enderlein, Rolf Krahl, Uwe Ortmann, Michael Wahl, Rainer Erdmann, and Edgar O. Klose "Lifetime-based identification of single molecules", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208465

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