Left and right topography of F-actin filaments

Leda Chang; Fransiska S. Franke; Paula Flicker; David Keller

doi:10.1117/12.205937

30 March 1995 Left and right topography of F-actin filaments

Leda Chang, Fransiska S. Franke, Paula Flicker, David Keller

Proceedings Volume 2384, Scanning Probe Microscopies III; (1995) https://doi.org/10.1117/12.205937
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

F-actin filaments have been found to adopt conformations with both right handed and left handed topographies, as revealed by AFM imaging in air. The left handed conformation has not been observed previously by either AFM or electron microscopy. Either handedness can be produced by controlling the conditions under which the molecules are deposited for imaging by Scanning Force Microscopy or Transmission Electron Microscopy. Low salt conditions favor the left handed form, and high salt conditions favor the right handed form. The ability of F-actin to undergo larger changes may still reflect smaller changes that take place under physiological conditions.

Citation Download Citation

Leda Chang, Fransiska S. Franke, Paula Flicker, and David Keller "Left and right topography of F-actin filaments", Proc. SPIE 2384, Scanning Probe Microscopies III, (30 March 1995); https://doi.org/10.1117/12.205937

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