Study of structure and functional states of alcohol oxidase by surface-enhanced Raman spectroscopy

Nataliya D. Strekal; Sergei A. Maskevich; Irina M. Artsukevich; Leonid N. Kivach; Ivan P. Chernikevich

doi:10.1117/12.197441

2 January 1995 Study of structure and functional states of alcohol oxidase by surface-enhanced Raman spectroscopy

Nataliya D. Strekal, Sergei A. Maskevich, Irina M. Artsukevich, Leonid N. Kivach, Ivan P. Chernikevich

Proceedings Volume 2370, 5th International Conference on Laser Applications in Life Sciences; (1995) https://doi.org/10.1117/12.197441
Event: Laser Applications in Life Sciences: 5th International Conference, 1994, Minsk, Belarus

Abstract

The SERS spectra of alcohol oxidase purified from Pichia Pastoris and Candida Boidinii adsorbed on silver electrode were obtained. The effect of stabilization of protein by NaN₃ was studied by SERS of flavin adenine dinucleitide released from flavoprotein near the Ag surface. The dissociation of falvin from flavoprotein in N3-anion from and in neutral form independence on functional state of protein has been supposed. The association of protein oligomers and the peculiarities of quaternary structure are discussed as a reason of different SERS behavior of alcohol oxidase from various different sources.

Citation Download Citation

Nataliya D. Strekal, Sergei A. Maskevich, Irina M. Artsukevich, Leonid N. Kivach, and Ivan P. Chernikevich "Study of structure and functional states of alcohol oxidase by surface-enhanced Raman spectroscopy", Proc. SPIE 2370, 5th International Conference on Laser Applications in Life Sciences, (2 January 1995); https://doi.org/10.1117/12.197441

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