Spectral fluorescence and scattering of cyanobacteria and diatoms held by optical tweezers

Gregory J. Sonek; Yagang Liu; Rodolfo H. Iturriaga

doi:10.1117/12.190051

26 October 1994 Spectral fluorescence and scattering of cyanobacteria and diatoms held by optical tweezers

Gregory J. Sonek, Yagang Liu, Rodolfo H. Iturriaga

Proceedings Volume 2258, Ocean Optics XII; (1994) https://doi.org/10.1117/12.190051
Event: Ocean Optics XII, 1994, Bergen, Norway

Abstract

Optical tweezers is a term used to describe the optical force generation and confinement process by a highly focused laser beam. The forces exerted by the tweezer are sufficient to confine and move cells and particles without physical contact. When integrated with fluorescence or scattering detection, the laser tweezer can become a powerful instrument for the rapid characterization of the optical properties of isolated organic marine particulates and phytoplanktonic cells, from which bulk properties may be inferred. This technique offers the advantage of studying planktonic cells and organisms in their natural environment by confinement without immobilization, thereby preserving the spectral absorption and fluorescence properties of the samples under study. Herein, we report, for the first time, on the measurement of the spectral fluorescence and scattering of cyanobacteria and diatoms which have been confined by an optical tweezer. Preliminary data shows the characteristic emission peak from the chlorophyll (alpha) pigment (685 nm) for both samples, as well as spectral features that may be related to other photosynthetic pigments.

Citation Download Citation

Gregory J. Sonek, Yagang Liu, and Rodolfo H. Iturriaga "Spectral fluorescence and scattering of cyanobacteria and diatoms held by optical tweezers", Proc. SPIE 2258, Ocean Optics XII, (26 October 1994); https://doi.org/10.1117/12.190051

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