Instrumental considerations for deriving spectral photosynthetic absorption coefficients from total phytoplankton absorption

Mary E. Culver; Richard F. Davis; Mary Jane Perry

doi:10.1117/12.190055

26 October 1994 Instrumental considerations for deriving spectral photosynthetic absorption coefficients from total phytoplankton absorption

Mary E. Culver, Richard F. Davis, Mary Jane Perry

Author Affiliations +

Proceedings Volume 2258, Ocean Optics XII; (1994) https://doi.org/10.1117/12.190055
Event: Ocean Optics XII, 1994, Bergen, Norway

Abstract

The phytoplankton absorption coefficient is primarily a function of absorption by photosynthetic and photoprotective pigments. Absorption by photosynthetic pigments can be separated from that by other pigments by the former's ability to induce chlorophyll (alpha) (chl (alpha) ) fluorescence. Chl (alpha) fluoresces at wavelengths between 660 and 760 nm with a distinct peak at 683 nm and a broad shoulder near 730 nm. The use of Basic Blue 3 as a quantum counter in spectrofluorimetry allows measurement of chl (alpha) fluorescence at 730 nm and hence, determination of phytoplankton fluorescence excitation spectra between 380 and 700 nm. Extension of measurements beyond 600 nm, the limit of the conventional quantum counter rhodamine B, allows direct comparison of the total absorption spectrum and the fluorescence excitation spectrum between 380 and 700 nm. Errors in the measurement of both types of spectra must be identified and minimized for reliable interpretation of either spectrum. Factors of primary importance include instrument-specific correction spectra for spectrofluorimeter configurations, accuracy in the absorption measurement, and sample handling.

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Mary E. Culver, Richard F. Davis, and Mary Jane Perry "Instrumental considerations for deriving spectral photosynthetic absorption coefficients from total phytoplankton absorption", Proc. SPIE 2258, Ocean Optics XII, (26 October 1994); https://doi.org/10.1117/12.190055

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