New waves in light microscopy

Daniel L. Farkas; Brent Bailey; Frederick Lanni; D. Lansing Taylor

doi:10.1117/12.182704

17 August 1994 New waves in light microscopy

Daniel L. Farkas, Brent Bailey, Frederick Lanni, D. Lansing Taylor

Proceedings Volume 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV; (1994) https://doi.org/10.1117/12.182704
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

The light microscope is an icon of the sciences, but its sophistication and usefulness have had a far from linear evolution. During the last decade great progress in fluorescent probe design, light source and detector technology, and computers have contributed to a revolutionary increase in the capabilities of light microscopy, turning it from a static, 2D tool into a dynamic, 3D means of studying live biological specimens. We believe that the next wave in light microscopy is based on automation and improved spatio-temporal resolution. Our work on multimode robotic microscopy and standing wave-excited fluorescence microscopy is presented here. The former allows simultaneous imaging with several modes of light microscopy and good specimen control (temperature, displacement, chemistry), while the latter affords an axial resolution significantly improving on confocal microscopy and, for the first time, exceeding transverse resolution. Examples of biological information obtainable solely by these advances are also given.

Citation Download Citation

Daniel L. Farkas, Brent Bailey, Frederick Lanni, and D. Lansing Taylor "New waves in light microscopy", Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); https://doi.org/10.1117/12.182704

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available