Investigation of optic nerve development using neuronal tracing dyes

James L. Lambert; Mark S. Borchert M.D.

doi:10.1117/12.182752

17 August 1994 Investigation of optic nerve development using neuronal tracing dyes

James L. Lambert, Mark S. Borchert M.D.

Proceedings Volume 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV; (1994) https://doi.org/10.1117/12.182752
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

Numerical techniques are described which allow quantitative interpretation of 2D and 3D histological imagery obtained from tissue samples which are stained with the neuronal tracer Fast Blue. The methods illustrated in this investigation are readily extensible to many different types of micro neuro-anatomy, and are particularly well suited for investigating pharmacological or environmental perturbations on neuron development. These techniques were used to quantitatively describe neurological development of the optic nerve in the pigmented rat. This analysis was used to show that most of the significant refinement in the retinotectal map occurs during the second to fourth week of life and with coincident with eye opening.

Citation Download Citation

James L. Lambert and Mark S. Borchert M.D. "Investigation of optic nerve development using neuronal tracing dyes", Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); https://doi.org/10.1117/12.182752

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available