Single-mode tapered optical fiber loop immunosensor II: assay of anti-cholera toxin immunoglobulins

Robert S. Marks; Zoe M. Hale; Myron M. Levine; C. R. Lowe; Frank P. Payne

doi:10.1117/12.180747

28 July 1994 Single-mode tapered optical fiber loop immunosensor II: assay of anti-cholera toxin immunoglobulins

Robert S. Marks, Zoe M. Hale, Myron M. Levine, C. R. Lowe, Frank P. Payne

Proceedings Volume 2131, Biomedical Fiber Optic Instrumentation; (1994) https://doi.org/10.1117/12.180747
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

An evanescent wave immunoassay for cholera antitoxin immunoglobulins was performed using a single mode tapered optical fiber loop sensor. The transducer was silanized with 3- glycidoxypropyltrimethoxysilane and chemically modified to link covalently either cholera toxin B subunit or a synthetic peptide derived from it, CTP3. The sensor was exposed to seral fluids, obtained from human volunteers having been exposed to live virulent Vibrio cholerae 01 and shown to produce rice-water stools. Other toxins of interest, such as Clostridium botulinum toxin A, have been tested on similar systems. The bound unlabelled immunoglobulins were then exposed to a mixture of FITC-anti-IgG and TRITC-anti-IgA, without requirement for a separation step. The emanating fluorescent emissions of fluorescein and rhodamine, excited by the input laser light, were coupled back into the guided mode of the tapered fiber, and used to determine the concentrations of the complementary antigens.

Citation Download Citation

Robert S. Marks, Zoe M. Hale, Myron M. Levine, C. R. Lowe, and Frank P. Payne "Single-mode tapered optical fiber loop immunosensor II: assay of anti-cholera toxin immunoglobulins", Proc. SPIE 2131, Biomedical Fiber Optic Instrumentation, (28 July 1994); https://doi.org/10.1117/12.180747

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