Novel fluorescence method for development of nicotinamide adenine dinucleotide and secondary biosensors

Ashutosh Sharma

doi:10.1117/12.180768

28 July 1994 Novel fluorescence method for development of nicotinamide adenine dinucleotide and secondary biosensors

Ashutosh Sharma

Proceedings Volume 2131, Biomedical Fiber Optic Instrumentation; (1994) https://doi.org/10.1117/12.180768
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

Photon fusion is proposed as a new method for molecular sensor development. In a molecular system, higher wavelength radiation is absorbed, and a lower wavelength radiation is emitted that is quenched by an analyte. This concept is demonstrated by developing a sensitive assay for reduced nicotinamide adenine dinucleotide (NADH). Indicator benzopurpurin 4B, when excited at 458 nm, emits at 357 nm. This latter fluorescence is quenched extremely efficiently by NADH, with a half quenching concentration of 40 (mu) M and a Stern-Volmer constant of 25 X 10³ M^-1. The advantages of this method are outlined.

Citation Download Citation

Ashutosh Sharma "Novel fluorescence method for development of nicotinamide adenine dinucleotide and secondary biosensors", Proc. SPIE 2131, Biomedical Fiber Optic Instrumentation, (28 July 1994); https://doi.org/10.1117/12.180768

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