Comparison of laser-activated tissue solders and thrombin-activated cryoprecipitate for wound closure

Mark L. Kayton; Steven K. Libutti M.D.; Marc Bessler; John D. F. Allendorf; Simon D. Eiref; Gerard Marx; Xiaode Mou M.D.; Alfredo M. Morales; Michael R. Treat M.D.; Roman Nowygrod M.D.

doi:10.1117/12.184939

7 September 1994 Comparison of laser-activated tissue solders and thrombin-activated cryoprecipitate for wound closure

Mark L. Kayton, Steven K. Libutti M.D., Marc Bessler, John D. F. Allendorf, Simon D. Eiref, Gerard Marx, Xiaode Mou M.D., Alfredo M. Morales, Michael R. Treat M.D., Roman Nowygrod M.D.

Author Affiliations +

Proceedings Volume 2128, Laser Surgery: Advanced Characterization, Therapeutics, and Systems IV; (1994) https://doi.org/10.1117/12.184939
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

To determine the relative strengths of various biologic adhesives at several timepoints, we compared thrombin-activated SD (solvent-detergent treated) cryoprecipitate with laser- activated SD cryoprecipitate and a laser-activated, albumin-based glue. Male Sprague-Dawley rats (n equals 79) received four, 3-cm, dorsal skin incisions which were closed with either laser- activated cryoprecipitate, laser-activated albumin solder, thrombin-activated cryoprecipitate, or standard skin staples. The cryoprecipitate was derived from pooled human plasma and was treated with a solvent-detergent process, rendering it free of envelope-coated viruses (i.e., HBV, HIV). An 808-nm diode laser was used to activate each solder with an average duration of exposure of 75 seconds per incision. Animals were sacrificed for evaluation of wound tensile strength and histology at 0 hours, 2 hours, 4 hours, and 4 days. At all timepoints tested, laser-activated solders were significantly stronger than thrombin-activated cryoprecipitate (p < 0.03) and control wounds (p < 0.003). There was no significant difference in tensile strength between the two types of laser-activated solder at any timepoint.

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Mark L. Kayton, Steven K. Libutti M.D., Marc Bessler, John D. F. Allendorf, Simon D. Eiref, Gerard Marx, Xiaode Mou M.D., Alfredo M. Morales, Michael R. Treat M.D., and Roman Nowygrod M.D. "Comparison of laser-activated tissue solders and thrombin-activated cryoprecipitate for wound closure", Proc. SPIE 2128, Laser Surgery: Advanced Characterization, Therapeutics, and Systems IV, (7 September 1994); https://doi.org/10.1117/12.184939

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