Fluorescence studies of 5-aminolaevulinic acid induced protoporphyrin IX and sulphonated aluminium phthalocyanine tissue distributions

Alexander J. MacRobert; M. D. Judd; Jaroslaw Regula; C. S. Loh; Stephen G. Brown

doi:10.1117/12.166836

15 January 1994 Fluorescence studies of 5-aminolaevulinic acid induced protoporphyrin IX and sulphonated aluminium phthalocyanine tissue distributions

Alexander J. MacRobert, M. D. Judd, Jaroslaw Regula, C. S. Loh, Stephen G. Brown

Author Affiliations +

Proceedings Volume 2081, Optical Biopsy; (1994) https://doi.org/10.1117/12.166836
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

A quantitative technique based on fluorescence microscopic detection has been developed for measurements of photosensitizer distributions in frozen tissue sections using a highly sensitive charge-coupled device camera. Studies have been carried out on sulphonated aluminum phthalocyanine and Protoporphyrin IX induced by administration of exogenous 5-aminolaevulinic acid. This technique has enabled microscopic pharmacokinetic studies to be performed in a range of normal tissues and tumors in order to improve our understanding of the photosensitization mechanism and enable optimal treatment times and dosimetry to be determined. Comparisons with chemical extraction data are reviewed and the feasibility of using biopsies from patients for optimizing dosimetry is discussed.

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Alexander J. MacRobert, M. D. Judd, Jaroslaw Regula, C. S. Loh, and Stephen G. Brown "Fluorescence studies of 5-aminolaevulinic acid induced protoporphyrin IX and sulphonated aluminium phthalocyanine tissue distributions", Proc. SPIE 2081, Optical Biopsy, (15 January 1994); https://doi.org/10.1117/12.166836

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