Diffusion of single molecules through a Gaussian laser beam

Rudolf Rigler; Ulo Mets

doi:10.1117/12.146154

17 June 1993 Diffusion of single molecules through a Gaussian laser beam

Rudolf Rigler, Ulo Mets

Proceedings Volume 1921, Laser Spectroscopy of Biomolecules; (1993) https://doi.org/10.1117/12.146154
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

The diffusion of a single molecule through a sharply focused laser beam has been observed. The diffusion was observed by recording photoelectron bursts of single molecules in transit through the laser beam with Gaussian intensity profile. Up to 20 photoelectrons were detected for Rhodamin 6G in a transit time of 40 .ts which corresponds to the diffusion time through a volume element of 2x10'61. From the intensity correlation the probability for the occurence of one molecule in the volume element of observation is determined. The existence of one molecule events is also verified by the measured and calculated photoelectron number distribution.

Citation Download Citation

Rudolf Rigler and Ulo Mets "Diffusion of single molecules through a Gaussian laser beam", Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); https://doi.org/10.1117/12.146154

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