Unbiased sampling for 3-D measurements in serial sections

Mark Anthony Browne; Hong Qiang Zhao; Vyvyan Howard; Glenn D. Jolleys

doi:10.1117/12.59590

26 June 1992 Unbiased sampling for 3-D measurements in serial sections

Mark Anthony Browne, Hong Qiang Zhao, Vyvyan Howard, Glenn D. Jolleys

Proceedings Volume 1660, Biomedical Image Processing and Three-Dimensional Microscopy; (1992) https://doi.org/10.1117/12.59590
Event: SPIE/IS&T 1992 Symposium on Electronic Imaging: Science and Technology, 1992, San Jose, CA, United States

Abstract

Previous studies have shown that the `disector' is a 3-D probe which can sample particles uniformly in 3-D space, and that the mean characteristics of the disector sample is an unbiased estimate of the same mean characteristics of all the particles in the reference space, if the disector is `uniform randomly' probed in the space. But to date the technique has been limited to manual operations for particle counting. As a 3-D sampling tool, its potential has not been fully realized due to the difficulties mainly associated with its manual implementation and the subsequent measurements on the resultant disector sample. In this paper, we present an image processing algorithm which can establish a disector sample of convex particles from a set of serial sections. The algorithm enables the automatic implementation of Cavalieri's principle in the environment of an image analyzer and may therefore lead to a direct measurement of particle size distribution.

Citation Download Citation

Mark Anthony Browne, Hong Qiang Zhao, Vyvyan Howard, and Glenn D. Jolleys "Unbiased sampling for 3-D measurements in serial sections", Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); https://doi.org/10.1117/12.59590

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available