Individual in-vitro sensitivities of human pancreatic carcinoma cell lines to photodynamic therapy

K. Thomas Moesta; Andrew Dmytrijuk; Peter M. Schlag; Thomas S. Mang

doi:10.1117/12.60926

1 June 1992 Individual in-vitro sensitivities of human pancreatic carcinoma cell lines to photodynamic therapy

K. Thomas Moesta, Andrew Dmytrijuk, Peter M. Schlag, Thomas S. Mang

Proceedings Volume 1645, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy; (1992) https://doi.org/10.1117/12.60926
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

Photodynamic therapy (PDT) is a promising alternative in the treatment of pancreatic cancer in man, due to the low sensitivity of the normal pancreas to PDT as shown in preclinical studies. Investigations on four human pancreatic cancer lines (MIA PaCa-2, PaCa 1, PaCa 3, and CAPAN 2) in vitro demonstrated a considerable variety in PDT-sensitivity proportional to the degree of differentiation, which was related to photosensitizer-uptake (PhotofrinTM). The well differentiated pancreatic tumor line Capan 2 showed a close relationship between high cell density and increased PDT-resistance. The Photofrin uptake of Capan 2 at high cell densities could be increased by short trypsinization prior to photosensitizer exposure. The data supports the hypothesis that a complex intercellular organization reduces the cell surface available for photosensitizer uptake and may cause the relative PDT resistance of normal pancreatic tissues and highly differentiated tumors.

Citation Download Citation

K. Thomas Moesta, Andrew Dmytrijuk, Peter M. Schlag, and Thomas S. Mang "Individual in-vitro sensitivities of human pancreatic carcinoma cell lines to photodynamic therapy", Proc. SPIE 1645, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy, (1 June 1992); https://doi.org/10.1117/12.60926

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