Effect of molecular ordering on distance distributions of flexible donor-acceptor pairs

Ignacy Gryczynski; Zygmunt Gryczynski; Wieslaw M. Wiczk; Jozef Kusba; Joseph R. Lakowicz

doi:10.1117/12.58257

1 April 1992 Effect of molecular ordering on distance distributions of flexible donor-acceptor pairs

Ignacy Gryczynski, Zygmunt Gryczynski, Wieslaw M. Wiczk, Jozef Kusba, Joseph R. Lakowicz

Proceedings Volume 1640, Time-Resolved Laser Spectroscopy in Biochemistry III; (1992) https://doi.org/10.1117/12.58257
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

Measurements of time-resolved intramolecular energy transfer in progressively stretched poly(vinyl alcohol) films were performed. The donor (tryptophan) and acceptor (dansyl) were linked with flexible polymethylene chain. Distance distributions were recovered from frequency-domain measurements of the donor decay. In isotropic PVA (in solution) a wide range of distances were detected (Gaussian full width at half maximum of about 16 angstroms) with an average distance of 13 angstroms. The donor-acceptor distance distribution became progressively more narrow when the PVA films were stretched. Four-fold or more stretching results in a single donor-acceptor distance. The maximal measured donor-acceptor distance of 23 angstroms is in excellent agreement with the computed distance for the fully stretched conformation. The possibility of partial and/or full ordering of polymethylene chains by stretching the PVA films can also be useful in the study of other distance-dependent interactions, such as electron transfer or distance-dependent quenching.

Citation Download Citation

Ignacy Gryczynski, Zygmunt Gryczynski, Wieslaw M. Wiczk, Jozef Kusba, and Joseph R. Lakowicz "Effect of molecular ordering on distance distributions of flexible donor-acceptor pairs", Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); https://doi.org/10.1117/12.58257

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