Dilemma of correlating fluorescence quantum yields and intensity decay times in single-tryptophan mutant proteins

Arthur G. Szabo; C. Faerman

doi:10.1117/12.58202

1 April 1992 Dilemma of correlating fluorescence quantum yields and intensity decay times in single-tryptophan mutant proteins

Arthur G. Szabo, C. Faerman

Author Affiliations +

Proceedings Volume 1640, Time-Resolved Laser Spectroscopy in Biochemistry III; (1992) https://doi.org/10.1117/12.58202
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

The relationship between the fluorescence quantum yield, (phi) f, radiative lifetime, (tau) r, and excited singlet state lifetime, (tau) s, is considered to be fundamental in fluorescence studies. Because of advances in instrumentation and data analysis the intensity decay times of tryptophan in proteins can be determined with a high degree of confidence and accuracy. Site directed mutagenesis allows one to place tryptophan residues in different locations within proteins. In several mutant single tryptophan containing proteins studied in our laboratory it has often been noted that there is a lack of a proportional correlation between the measured fluorescence quantum yield and the mean fluorescence decay time. This data and examples from the literature are presented and rationalizations are discussed. The intent is to stimulate experimental design and theoretical studies. This will lead to new intuitions from fluorescence studies of mutant proteins regarding the interrelationship of their structure, dynamics, and function.

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Arthur G. Szabo and C. Faerman "Dilemma of correlating fluorescence quantum yields and intensity decay times in single-tryptophan mutant proteins", Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); https://doi.org/10.1117/12.58202

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