Research on depth enhancement of fluorescence imaging based on adaptive optics

Haibo Yang; Tianxuan Feng; Ronghua Fan; Hanyi Zhang; Rong Fan; Yin Cao; Lijing Li

doi:10.1117/12.3045589

26 September 2024 Research on depth enhancement of fluorescence imaging based on adaptive optics

Haibo Yang, Tianxuan Feng, Ronghua Fan, Hanyi Zhang, Rong Fan, Yin Cao, Lijing Li

Proceedings Volume 13282, Second Advanced Imaging and Information Processing Conference (AIIP 2024); 132820D (2024) https://doi.org/10.1117/12.3045589
Event: Second Advanced Imaging and Information Processing Conference (AIIP 2024), 2024, Xining, China

Abstract

Fluorescence imaging is one of the basic research of biological microscope imaging, and improving the imaging depth of biological microscopes has always been a research hotspot . One of the factors affecting the depth is that the excitation light will be absorbed and scattered by the tissue and cannot be focused on the target fluorescent area. Therefore, this study uses an adaptive optics scheme to correct the wavefront phase of the excitation light, and uses the intensity of fluorescence as a quantitative parameter. The results show that the research scheme in this paper has a good effect on improving the quality of deep imaging.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Haibo Yang, Tianxuan Feng, Ronghua Fan, Hanyi Zhang, Rong Fan, Yin Cao, and Lijing Li "Research on depth enhancement of fluorescence imaging based on adaptive optics", Proc. SPIE 13282, Second Advanced Imaging and Information Processing Conference (AIIP 2024), 132820D (26 September 2024); https://doi.org/10.1117/12.3045589

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