Rapid volumetric imaging utilizing structured light beams

Yuichi Kozawa

doi:10.1117/12.3035429

22 November 2024 Rapid volumetric imaging utilizing structured light beams

Yuichi Kozawa

Proceedings Volume 13240, Holography, Diffractive Optics, and Applications XIV; 1324005 (2024) https://doi.org/10.1117/12.3035429
Event: SPIE/COS Photonics Asia, 2024, Nantong, Jiangsu, China

Abstract

Structured light beams, with unique spatial properties, are applied to rapid three-dimensional (3D) imaging in laser scanning microscopy. Our proposed method utilizes a needle-shaped excitation spot and wavefront modulation of fluorescence signals to retrieve depth information from a single raster scan. The combined use of these techniques eliminates the need for multiple focal plane acquisitions, significantly improving 3D image acquisition speed. The effectiveness of this technique was demonstrated by capturing the 3D Brownian motion of nanoparticles. This approach holds promise for rapid 3D volumetric imaging in various fluorescence microscopy applications.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Yuichi Kozawa "Rapid volumetric imaging utilizing structured light beams", Proc. SPIE 13240, Holography, Diffractive Optics, and Applications XIV, 1324005 (22 November 2024); https://doi.org/10.1117/12.3035429

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available