Three-dimensional imaging in microscopy

S. Kessler; Rosemarie Hild; G. Nitzche; Jork Hebenstreit

doi:10.1117/12.34812

1 July 1990 Three-dimensional imaging in microscopy

S. Kessler, Rosemarie Hild, G. Nitzche, Jork Hebenstreit

Proceedings Volume 1319, Optics in Complex Systems; (1990) https://doi.org/10.1117/12.34812
Event: 15th International Optics in Complex Systems, 1990, Garmisch, Germany

Abstract

A theory of 3D- imaging of a thick object with a depth h (Fig.l ) is developed for vertical Illumination microscopy (Fig2). The amount as well as the phase of the Fourier spectrum which corresponds to the 3D- object is discussed in detaiL Fig3 shows the amount tT(01 of the Fourier spectrum for different object depth. ii (V I has zercs In the case of planar object (h0) . As the thickness of the object is increased an enhancement of the spatial frequencies and a shift of the Ninhia occurs. The zeri of the planar object spectrum are lsed.

Citation Download Citation

S. Kessler, Rosemarie Hild, G. Nitzche, and Jork Hebenstreit "Three-dimensional imaging in microscopy", Proc. SPIE 1319, Optics in Complex Systems, (1 July 1990); https://doi.org/10.1117/12.34812

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