Fast optical diffraction tomography microscopy with large field of view and lossless pupil beam combination

Wanxue Wei; Dashan Dong; Kebin Shi

doi:10.1117/12.3028648

2 October 2024 Fast optical diffraction tomography microscopy with large field of view and lossless pupil beam combination

Wanxue Wei, Dashan Dong, Kebin Shi

Author Affiliations +

Proceedings Volume 13139, Ultrafast Nonlinear Imaging and Spectroscopy XII; 1313910 (2024) https://doi.org/10.1117/12.3028648
Event: Optical Engineering + Applications, 2024, San Diego, California, United States

Conference Poster

Abstract

Optical diffractive tomography (ODT) microscopy is a normal wide-field, non-invasive and label-free three-dimensional imaging technology for cells and tissues. The traditional ODT microscopy has a little field-of-view (FOV) of about 80um, which needs to be reconstructed by regions and then spliced for large complex biological samples with sub-millimeter scale. However, there is interference of ringing effect during splicing, which limits its application in sub-millimeter biological samples. In this paper, an ODT microscopy with a wider FOV is proposed. The FOV is 196um, more than three times that of conventional technology, and the photon flux is higher. The results show that the wider-field ODT microscopy has better imaging performance, higher signal-to-noise ratio on the sub-millimeter samples without splicing.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Wanxue Wei, Dashan Dong, and Kebin Shi "Fast optical diffraction tomography microscopy with large field of view and lossless pupil beam combination", Proc. SPIE 13139, Ultrafast Nonlinear Imaging and Spectroscopy XII, 1313910 (2 October 2024); https://doi.org/10.1117/12.3028648

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