Ultra-compact fluorescence microscope for life-sciences

S. Moreno; A. Vilà; J. Ramón-Azcón; J. D. Prades; A. Diéguez

doi:10.1117/12.3016292

17 June 2024 Ultra-compact fluorescence microscope for life-sciences

S. Moreno, A. Vilà, J. Ramón-Azcón, J. D. Prades, A. Diéguez

Proceedings Volume 13024, Optical Instrument Science, Technology, and Applications III; 130240D (2024) https://doi.org/10.1117/12.3016292
Event: SPIE Optical Systems Design, 2024, Strasbourg, France

Abstract

Miniature microscopy provides a transformative approach to observe objects and enable continuous monitoring with ultra-compact microscopes attached directly to specimens, facilitating parallel analysis. This innovation is particularly valuable for applications such as drug discovery using organ-on-a-chip devices, which require the assessment of numerous drug/sample pairs prior to clinical trials. Ultra-compact microscopes were previously limited to brightfield techniques, which prevented the use of powerful tools like fluorescent microscopy. In this work, we present a miniature microscope with integrated fluorescence measurement capabilities. This microscope consists of a custom chip with a 10 μm-diameter single-photon avalanche diode (SPAD) faced to a 640 × 480 InGaN/GaN 4 μm-pitch LED microdisplay. It operates in raster mode, activating individual LEDs to map specimens in 2D while measuring fluorescence light with the SPAD chip. Our results demonstrate its suitability for life sciences imaging. For example, we observed a muscle-ona-chip stained with Alexa Fluor 488 to study drug efficacy on sarcopenia. Furthermore, these microscopes exhibit superior speed compared to the previously reported ultra-compact brightfield microscopes, achieving a 240-fold increase in imaging rate by means of hardware controller integration on FPGA.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

S. Moreno, A. Vilà, J. Ramón-Azcón, J. D. Prades, and A. Diéguez "Ultra-compact fluorescence microscope for life-sciences", Proc. SPIE 13024, Optical Instrument Science, Technology, and Applications III, 130240D (17 June 2024); https://doi.org/10.1117/12.3016292

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available