Single cell analysis by holographic microscopy and flow cytometry: an high-throughput and label-free approach

Giusy Giugliano; Michela Schiavo; Daniele Pirone; Vittorio Bianco; Pasquale Memmolo; Lisa Miccio; Pietro Ferraro

doi:10.1117/12.3023928

20 June 2024 Single cell analysis by holographic microscopy and flow cytometry: an high-throughput and label-free approach

Giusy Giugliano, Michela Schiavo, Daniele Pirone, Vittorio Bianco, Pasquale Memmolo, Lisa Miccio, Pietro Ferraro

Author Affiliations +

Proceedings Volume 13008, Biophotonics in Point-of-Care III; 130080F (2024) https://doi.org/10.1117/12.3023928
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Combining Digital Holography and Flow Cytometry, a powerful imaging technique which offers an effective approach in biomedicine investigations is obtained. Indeed, the extension of phase-contrast tomography cells in microfluidic environments allows for high-throughput property addressing the rising need for realistic and comprehensive datasets. This is achieved through the rapid collection of numerous flowing and rotating cell populations by refraining from using dyes or fluorescent markers. Cells’ roto-translation motion produces holographic interference patterns which are recorded to allow implementation of the computational procedure that provides reconstructed two-dimensional phase quantitative maps which contain the whole information from the biological specimen, such as morphological biophysical properties. The capacity to examine the same biological sample from multiple perspectives enables complete label-free analysis, an accomplishment made possible through the application of holographic tomography technology. This advanced technology stands at the forefront of single-cell biological investigation. Here, we show the potential of this technique in different biomedical applications. Indeed, effectiveness of this imaging method is highlighted in identifying and analyzing cancer cells, e.g circulating tumor cells, diagnosing blood-related disorders, classifying distinct cell lines within diverse populations, and identifying intricate internal cellular microstructures. Moreover, this innovative approach hints at promising future applications across different domains, including liquid biopsy, addressing drug resistance, and understanding genetic anomalies.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Giusy Giugliano, Michela Schiavo, Daniele Pirone, Vittorio Bianco, Pasquale Memmolo, Lisa Miccio, and Pietro Ferraro "Single cell analysis by holographic microscopy and flow cytometry: an high-throughput and label-free approach", Proc. SPIE 13008, Biophotonics in Point-of-Care III, 130080F (20 June 2024); https://doi.org/10.1117/12.3023928

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