Determining molar extinction coefficient by high-accuracy optical saturation measurements

Meelis-Mait Sildoja; Jüri Pahapill; Charles W. Stark; Aleksander Rebane

doi:10.1117/12.3017221

20 June 2024 Determining molar extinction coefficient by high-accuracy optical saturation measurements

Meelis-Mait Sildoja, Jüri Pahapill, Charles W. Stark, Aleksander Rebane

Proceedings Volume 13004, Nonlinear Optics and its Applications 2024; 130040Q (2024) https://doi.org/10.1117/12.3017221
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Conference Poster

Abstract

Linear spectrophotometric transmittance measurements are widely used for determining the value of molar extinction coefficient and other basic photophysical parameters of dissolved molecular species. However, such measurements usually require prior information about the molar concentration of the studied chromophores, which in many cases such as e.g. genetically encoded self-maturing species, is not readily available. Here we use wavelength-tunable femtosecond pulses to demonstrate that by performing high-accuracy measurement of small intensity-dependent changes induced in the sample transmittance due to absorption saturation we are able to estimate the extinction coefficient without prior knowledge of the concentration.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Meelis-Mait Sildoja, Jüri Pahapill, Charles W. Stark, and Aleksander Rebane "Determining molar extinction coefficient by high-accuracy optical saturation measurements", Proc. SPIE 13004, Nonlinear Optics and its Applications 2024, 130040Q (20 June 2024); https://doi.org/10.1117/12.3017221

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