Variability in plant cell water dynamics as a genetic discriminatory method using terahertz quantum cascade laser-based laser feedback interferometry

Urbi Kundu; Jari Torniainen; Karl Bertling; Mayuri Kashyap; Khushboo Singh; Bogdan C. Donose; Tim Gillespie; Dragan Indjin; Lianhe Li; Edmund H. Linfield; A. Giles Davies; Paul Dean; Aparajita Bandyopadhyay; Amartya Sengupta; Aleksandar D. Rakić

doi:10.1117/12.3002438

12 March 2024 Variability in plant cell water dynamics as a genetic discriminatory method using terahertz quantum cascade laser-based laser feedback interferometry

Urbi Kundu, Jari Torniainen, Karl Bertling, Mayuri Kashyap, Khushboo Singh, Bogdan C. Donose, Tim Gillespie, Dragan Indjin, Lianhe Li, Edmund H. Linfield, A. Giles Davies, Paul Dean, Aparajita Bandyopadhyay, Amartya Sengupta, Aleksandar D. Rakić

Author Affiliations +

Proceedings Volume 12879, Photonic Technologies in Plant and Agricultural Science; 128790K (2024) https://doi.org/10.1117/12.3002438
Event: SPIE LASE, 2024, San Francisco, California, United States

Conference Poster

Abstract

In this article, we report our work on the development of a non- invasive, rapid, robust, and high-fidelity technique that can be used to discriminate between genetic variants. Our study focused on terahertz (THz) spectroscopy and imaging to distinguish between genetic variants of the Allium genus rapidly and accurately. This was done by measuring the cellular water dynamics of the samples by measuring their evaporation profiles using Laser Feedback Interferometry (LFI) with THz Quantum Cascade Lasers (QCL). The evaporation profiles of the samples were then processed to create trajectories in the amplitude-phase domain, which correlated with cell age, cell type, and the amount of water bound to biomolecules. This technique can differentiate between the members of the Allium genus. The presence of outliers was also studied to determine the effectiveness of the technique for different samples and to negate external influence. This was done to discern the extent of influence of cell biomechanics and biochemistry between genetic variants. We found that within a genus, different species would have different degree of interaction between cellular water and cell biochemistry, which could be clearly mapped out using THz-QCL-based LFI. Based on our observations, we propose that this method could be appropriate for observing minute alterations in cellular water dynamics in real-time, and in the future, has the potential to be employed for rapid and effective genetic discrimination in agricultural and genome conservation applications.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Urbi Kundu, Jari Torniainen, Karl Bertling, Mayuri Kashyap, Khushboo Singh, Bogdan C. Donose, Tim Gillespie, Dragan Indjin, Lianhe Li, Edmund H. Linfield, A. Giles Davies, Paul Dean, Aparajita Bandyopadhyay, Amartya Sengupta, and Aleksandar D. Rakić "Variability in plant cell water dynamics as a genetic discriminatory method using terahertz quantum cascade laser-based laser feedback interferometry", Proc. SPIE 12879, Photonic Technologies in Plant and Agricultural Science, 128790K (12 March 2024); https://doi.org/10.1117/12.3002438

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available