Hardware approach for comprehensive imaging of in vivo retina and in vitro retinal organoid using polarization-sensitive optical coherence tomography

S. Banerjee; M. Mandai; M. Nishida; T. Masuda; M. Eiraku; M. Okamoto; T. Nakashima; M. Yamanari; K. Totani; S. Sugiyama

doi:10.1117/12.3001180

12 March 2024 Hardware approach for comprehensive imaging of in vivo retina and in vitro retinal organoid using polarization-sensitive optical coherence tomography

S. Banerjee, M. Mandai, M. Nishida, T. Masuda, M. Eiraku, M. Okamoto, T. Nakashima, M. Yamanari, K. Totani, S. Sugiyama

Author Affiliations +

Proceedings Volume 12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; 128540O (2024) https://doi.org/10.1117/12.3001180
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Continuous monitoring of retinal pigment epithelium (RPE) cell growth and functionality is imperative in both pre- and post- RPE transplantation phases. We propose a telecentric add-on scan lens design that is integrated with the probe arm of a polarization-sensitive optical coherence tomography (PS-OCT) system for in vitro retinal organoid imaging. The system can be switched to in vivo or in vitro imaging mode depending on the requirement. In the context of retinal imaging, the polarization information of the RPE layer and other layers, along with their conventional morphology, can be effectively contrasted using the in vivo mode. The add-on lens attachment enables a wide field of view retinal organoid growth monitoring and its functionality visualization. It includes a field flattener to compensate for field curvature. The comprehensive PS-OCT system provides a 12 mm x 12 mm field of view on the retina and can cover up to 15 mm x 15 mm on retinal organoids. The system successfully images young and mature retinal organoids, capturing well-defined layered structures and highly pigmented outer RPE boundaries using polarimetric entropy contrast. Intensity based segmentation on the cross-section image of retinal organoid is performed to retrieve the thickness heatmap which provides the insight of organoids’ growth statistics.

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S. Banerjee, M. Mandai, M. Nishida, T. Masuda, M. Eiraku, M. Okamoto, T. Nakashima, M. Yamanari, K. Totani, and S. Sugiyama "Hardware approach for comprehensive imaging of in vivo retina and in vitro retinal organoid using polarization-sensitive optical coherence tomography", Proc. SPIE 12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, 128540O (12 March 2024); https://doi.org/10.1117/12.3001180

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