Multimodal fluorescence microscopy and 3D quantitative phase imaging/refractive index tomography in thick tissue samples

Zhenmin Li; Zhe Guang; Paloma Casteleiro Costa; Caroline Filan; Srinidhi Bharadwaj; Francisco E. Robles

doi:10.1117/12.3003242

12 March 2024 Multimodal fluorescence microscopy and 3D quantitative phase imaging/refractive index tomography in thick tissue samples

Zhenmin Li, Zhe Guang, Paloma Casteleiro Costa, Caroline Filan, Srinidhi Bharadwaj, Francisco E. Robles

Author Affiliations +

Proceedings Volume 12852, Quantitative Phase Imaging X; 128520I (2024) https://doi.org/10.1117/12.3003242
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Phase imaging and fluorescence microscopy provide valuable complementary information, and individually form the basis for a significant portion of the routing biological and biomedical optical imaging performed today. While multimodal phase and fluorescence microscopy has been explored for thin transparent samples to obtain structural information based on the refractive index distribution (with phase contrast) and molecular content (with fluorescence), combining these complementary technologies to study thick samples has been challenging and remains largely unexplored. This work presents the results of a study that combines quantitative phase imaging (QPI) and refractive index (RI) tomography in thick samples—using quantitative oblique back illumination—and bright field fluorescence deconvolution microscopy. The two technologies use a simple bright field microscope configuration with epi-illumination and through-focus z-stack acquisition, along with a deconvolution algorithm, to achieve 3D imaging. Phase and RI information is acquired nearly simultaneously with the fluorescence information with inherent co-registration of the two modalities. In this work, we will present the theoretical underpinning of this multimodal approach, describe the simple multimodal system, and show imaging results of thick tissues, such as labeled mice brains. This multimodal imaging approach could help biologists and clinicians gain a more comprehensive understanding of the tissue’s morphology and molecular composition, and can be widely applied across a number of biological and biomedical disciplines, including neuroscience, pathology, and oncology.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Zhenmin Li, Zhe Guang, Paloma Casteleiro Costa, Caroline Filan, Srinidhi Bharadwaj, and Francisco E. Robles "Multimodal fluorescence microscopy and 3D quantitative phase imaging/refractive index tomography in thick tissue samples", Proc. SPIE 12852, Quantitative Phase Imaging X, 128520I (12 March 2024); https://doi.org/10.1117/12.3003242

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available