Multiscale photoacoustic imaging of lymphatic vessels in rabbit ears with lymphedema

Takeshi Hirasawa; Masato Tsuchiya; Satoshi Kubo; Tomohiro Ishikawa; Ryuichi Azuma; Miya Ishihara

doi:10.1117/12.3005338

12 March 2024 Multiscale photoacoustic imaging of lymphatic vessels in rabbit ears with lymphedema

Takeshi Hirasawa, Masato Tsuchiya, Satoshi Kubo, Tomohiro Ishikawa, Ryuichi Azuma, Miya Ishihara

Proceedings Volume 12842, Photons Plus Ultrasound: Imaging and Sensing 2024; 1284210 (2024) https://doi.org/10.1117/12.3005338
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Lymphedema is tissue swelling caused by dysfunction of the lymphatic drainage system and is a common side effect after surgery and radiotherapy for malignant tumors. Preoperative lymphatic mapping is desired for lymphedema surgery. To date, several groups have applied photoacoustic (PA) imaging (PAI) using indocyanine green (ICG) to lymphedema patients for this purpose. PAI of lymphatic vessels is also useful for basic research using small animals, such as evaluating therapeutic techniques and analyzing tissue degeneration. To this end, we developed a PAI with a high-frequency linear array sensor that can visualize small lymph vessels in small animals. Furthermore, we have developed a PA microscopy (PAM) that can obtain cell-resolution images of surface tissues. PAI and PAM were tested by imaging lymphatic vessels in rabbit ears with lymphedema. PAI clearly visualized lymphatic vessels parallel to blood vessels. PAM was able to visualize ICG flowing back into the superficial lymph capillaries and dermis due to dermal backflow that occurs in lymphedema.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Takeshi Hirasawa, Masato Tsuchiya, Satoshi Kubo, Tomohiro Ishikawa, Ryuichi Azuma, and Miya Ishihara "Multiscale photoacoustic imaging of lymphatic vessels in rabbit ears with lymphedema", Proc. SPIE 12842, Photons Plus Ultrasound: Imaging and Sensing 2024, 1284210 (12 March 2024); https://doi.org/10.1117/12.3005338

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