Visualizing metabolites at the cellular level: advancements in 3-micron laser desorption mass spectrometry imaging

Robert T. Murray; Ronan A. Battle; Daniel Simon; Yuchen Xiang; Kenneth Robinson; Samuel F. Azevedo Magalhães; Yu Wang; Timothy H. Runcorn; Zoltan Takats

doi:10.1117/12.3008405

12 March 2024 Visualizing metabolites at the cellular level: advancements in 3-micron laser desorption mass spectrometry imaging

Robert T. Murray, Ronan A. Battle, Daniel Simon, Yuchen Xiang, Kenneth Robinson, Samuel F. Azevedo Magalhães, Yu Wang, Timothy H. Runcorn, Zoltan Takats

Author Affiliations +

Proceedings Volume 12836, Optical Biopsy XXII: Toward Real-Time Spectroscopic Imaging and Diagnosis; 1283602 (2024) https://doi.org/10.1117/12.3008405
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

By combining high beam quality picosecond pulsed optical parametric amplifiers at 2.94 μm with Rapid Evaportive Ionisation Mass Spectrometry (REIMS), we have demonstrated record spatial pixel resolutions for ambient mass spectrometry (MS) imaging of < 10 μm. In this contribution, we introduce our work in this area, demonstrating the platform workflow and highlighting recent results of metabolic imaging at the single cell resolution level.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Robert T. Murray, Ronan A. Battle, Daniel Simon, Yuchen Xiang, Kenneth Robinson, Samuel F. Azevedo Magalhães, Yu Wang, Timothy H. Runcorn, and Zoltan Takats "Visualizing metabolites at the cellular level: advancements in 3-micron laser desorption mass spectrometry imaging", Proc. SPIE 12836, Optical Biopsy XXII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 1283602 (12 March 2024); https://doi.org/10.1117/12.3008405

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