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Timely and effective detection of COVID-19 continues to be a critical aspect in managing the pandemic and setting the groundwork for future pathogen detection techniques. Present diagnostic technologies, especially those designed for POC (Point of Care) use in resource-constrained settings, must meet the evolving needs for rapidity, accuracy, and affordability. Within this context, preparing for pandemics necessitates the development of swift POC diagnostics. This research investigates the potential of the Arduino-driven LAMP (Loop-Mediated Isothermal Amplification) method for SARS-CoV-2 detection. An inexpensive, accessible, and portable "LAMP box" is proposed in this study, integrating the LAMP assay with an Arduino to create a potentially effective detection system for COVID-19. The device features a wireless technology network connecting all components. The reaction is performed at 65°C on an isothermal heating pad located beneath a solid aluminum base, providing a stable platform for reaction tubes and assays. Control of the entire system, powered via a 5-volt USB source, is provided through a built-in Bluetooth connection linked to an Arduino, facilitating control through a computer. The study concludes that such simple systems can effectively determine the presence or absence of artificial SARS-CoV-2 genetic material in samples. The presented research underscores the promise of Arduino-based LAMP technology, showcasing its specificity and sensitivity compared to the conventional RT-PCR (Reverse Transcription Polymerase Chain Reaction).
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