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Recent studies have highlighted the importance of understanding the architecture and function of microvasculature, and dysfunctions of these microvessels may underlie neurodegenerative disease. Here, we utilized a highly precision ultrafast laser-induced photothrombosis (PLP) method to occlude single capillaries and then quantitatively studied effects on vasodynamics and surrounding neurons. Analysis of the microvascular architecture and hemodynamics after single-capillary occlusion reveals distinct changes upstream vs downstream branches, which shows rapid regional flow redistribution and local downstream BBB leakage. Focal ischemia via capillary occlusions surrounding labeled target neuron induced dramatic and rapid lamina-specific changes in neuronal dendritic architecture. The adaptive changes in neuronal function networks were correlated to the degree of ischemia core. Further, we find that micro-occlusion at two different depths within the same vascular arbor results in distinct effects on flow profiles in layerin layer 2/3 vs layer 4. The current results raised the possibility that relatively greater impacts on microvascular function contribute to neuronal network function.
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