High-resolution optical imaging of infective endocarditis biofilms

Aaron Doug Deen; Antonio Lopez Marin; Gijs van Soest; Kirby Lattwein

doi:10.1117/12.3010254

13 March 2024 High-resolution optical imaging of infective endocarditis biofilms

Aaron Doug Deen, Antonio Lopez Marin, Gijs van Soest, Kirby Lattwein

Proceedings Volume 12819, Diagnostic and Therapeutic Applications of Light in Cardiology 2024; 128190U (2024) https://doi.org/10.1117/12.3010254
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Bacterial infections inside the heart, called infective endocarditis, result in high mortality. The bacteria encase themselves within a biofilm, which shields them from treatment by drugs or devices, and makes it challenging to diagnose and confirm infection, despite technological advances. In this study, we investigate the use of optical coherence microscopy (OCM), a non-invasive imaging modality, as a potential tool to visualize biofilms on heart valves. Biofilms were grown on porcine heart valves in human plasma using clinical isolates of Staphylococcus aureus or Streptococcus gordonii. S. aureus biofilms were treated with a fibrinolytic to degrade and remove biofilms. Valves were imaged before and after biofilm growth using OCM followed by subsequent confocal laser scanning microscopy using fluorescent staining. The resolutions and imaging areas of the two microscopes were matched. A comparative analysis of the two techniques showed that OCM can accurately differentiate between areas with and without biofilm. Our findings highlight OCM as a tool for non-contact, label free imaging that can provide key morphological information for infection diagnosis and therapy guidance.

Conference Presentation

Citation Download Citation

Aaron Doug Deen, Antonio Lopez Marin, Gijs van Soest, and Kirby Lattwein "High-resolution optical imaging of infective endocarditis biofilms", Proc. SPIE 12819, Diagnostic and Therapeutic Applications of Light in Cardiology 2024, 128190U (13 March 2024); https://doi.org/10.1117/12.3010254

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