Multiphoton tomography of human skin during oxygen inhalation

Karsten König; Isabell Wenskus; Aisada König

doi:10.1117/12.3000153

12 March 2024 Multiphoton tomography of human skin during oxygen inhalation

Karsten König, Isabell Wenskus, Aisada König

Proceedings Volume 12816, Photonics in Dermatology and Plastic Surgery 2024; 1281607 (2024) https://doi.org/10.1117/12.3000153
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Multiphoton tomography is a clinical imaging method to obtain high-resolution optical biopsies of human skin and to perform optical metabolic imaging (OMI) by time-correlated single photon counting / fluorescence lifetime imaging (FLIM) of autofluorescent coenzymes. We report on a long-term MPT-OMI study on two volunteers during oxygen treatment. Metabolic changes of epidermal skin cells have been recorded during daily two hours oxygen inhalation over an one-week-treatment period. Multimodal MPT (confocal reflection, autofluorescence, FLIM, SHG) has been performed with the tomograph MPTcompact based on a compact femtosecond fiber laser located inside a 360° imaging head mounted on a flexible mechanical arm. Imaging/tracking of specific intratissue cells could be performed with submicron resolution over two hours during oxygen inhalation. Oxygen inhalation influences the ratio free to bound NADH mainly in the basal cell layer close to the capillaries compared with the stratum granulosum. In conclusion, multiphoton tomography can be used for in vivo metabolic time-lapse high-resolution imaging of human skin during treatment, such as oxygen therapy.

Conference Presentation

Citation Download Citation

Karsten König, Isabell Wenskus, and Aisada König "Multiphoton tomography of human skin during oxygen inhalation", Proc. SPIE 12816, Photonics in Dermatology and Plastic Surgery 2024, 1281607 (12 March 2024); https://doi.org/10.1117/12.3000153

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