C-CLASS microscopy allows the detection of nanoscale changes in chromatin structure

Mark F. Coughlan; Giuseppe Pettinato; Maria Glyavina; Xuejun Zhang; Liming Chen; Umar Khan; Paul K. Upputuri; Yuri N. Zakharov; Lei Zhang; Le Qiu; Lev T. Perelman

doi:10.1117/12.2667044

9 August 2023 C-CLASS microscopy allows the detection of nanoscale changes in chromatin structure

Mark F. Coughlan, Giuseppe Pettinato, Maria Glyavina, Xuejun Zhang, Liming Chen, Umar Khan, Paul K. Upputuri, Yuri N. Zakharov, Lei Zhang, Le Qiu, Lev T. Perelman

Author Affiliations +

Proceedings Volume 12630, Advances in Microscopic Imaging IV; 126300U (2023) https://doi.org/10.1117/12.2667044
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

Coherent confocal light absorption and scattering spectroscopic (C-CLASS) microscopy, which extends the principles of light scattering spectroscopy to subcellular imaging, can be used to reveal biological structures well beyond the diffraction limit. Here we show that high-resolution C-CLASS microscopy can be used to detect nanoscale changes in chromatin structure. Unlike most methods for chromatin monitoring, C-CLASS microscopy can be used label-free in live cells. Live differentiating hiPSC organoids were measured over the space of sixteen days and characteristic chromatin changes were observed.

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Mark F. Coughlan, Giuseppe Pettinato, Maria Glyavina, Xuejun Zhang, Liming Chen, Umar Khan, Paul K. Upputuri, Yuri N. Zakharov, Lei Zhang, Le Qiu, and Lev T. Perelman "C-CLASS microscopy allows the detection of nanoscale changes in chromatin structure", Proc. SPIE 12630, Advances in Microscopic Imaging IV, 126300U (9 August 2023); https://doi.org/10.1117/12.2667044

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